Article

Triplex DNA and inverted repeats cause long-read sequencing bias against simple satellite DNA

2026-07-20

Abstract excerpt

We recently showed that Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio) have very strong sequencing bias against simple satellites, probably caused by single-stranded DNA folding into non-canonical (non-B) structures during sequencing. Here we extend these observations by computational and experimental approaches in the Drosophila and human genomes. We found that (i) only a small subset of sim...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f07df614-3e14-56e8-81dc-b4278630948f
DOI: 10.64898/2026.07.13.738322

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Triplex DNA and inverted repeats cause long-read sequencing bias against simple satellite DNADOI 10.64898/2026.07.13.738322Read

Comparative analysis of single-stranded and non-canonical DNA formation in human and other ape cells with telomere-to-telomere genomesSemantic proximity Read

Linking Dynamic DNA Secondary Structures to Genome InstabilitySemantic proximity Read

Distinct sequencing success at non-B-DNA motifsSemantic proximity Read

Slipped strand DNA structuresSemantic proximity Read

The implications of satellite DNA instability on cellular function and evolution.Semantic proximity Read

Microsatellites: simple sequences with complex evolution.Semantic proximity Read

High-Throughput Measurement of Metastable DNA Secondary Structures using Multiplexed Low-Yield Bisulfite Sequencing (MLB-seq)Semantic proximity Read

<i>In vivo</i> detection of DNA secondary structures using Permanganate/S1 Footprinting with Direct Adapter Ligation and Sequencing (PDAL-Seq)Semantic proximity Read

Examining coding structure and redundancy in DNA. How does DNA protect itself from life's uncertainty?Semantic proximity Read

A classical revival: Human satellite DNAs enter the genomics eraSemantic proximity Read

Evaluation of the correctable decoding sequencing as a new powerful strategy for DNA sequencing.Semantic proximity Read

Small Single-Stranded DNA Structure PredictionSemantic proximity Read