Article
A quantitative PCR assay for the detection and quantification of Shiga toxin-producing Escherichia coli (STEC) in minced beef and dairy products.
International journal of food microbiology - 15 Nov 2011
Derzelle S, Grine A, Madic J, de Garam C Peytavin, Vingadassalon N, Dilasser F, Jamet E, Auvray F
Abstract excerpt
Shiga toxin (Stx)-producing Escherichia coli (STEC) are amongst major causes of food-borne infectious diseases and outbreaks. A new quantitative PCR (qPCR) assay was designed to detect all known stx gene subtypes in a single reaction, including the most distant variant stx2f. Performance of this assay was evaluated in combination with two different internal amplification controls (IAC), a competitive one specific...
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