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Coupling multiplex pre-amplification and droplet digital PCR for longitudinal monitoring of <i>ESR1</i> and <i>PIK3CA</i> mutations from plasma cell-free DNA

2019-04-05

Abstract excerpt

Here we report on the development of a sensitive and cost-effective method to longitudinally track ESR1 and PIK3CA mutations from cfDNA in patients with metastatic breast cancer (MBC) using a streamlined and de-centralized workflow. Hotspot mutations in ESR1 have been shown to cause resistance to aromatase inhibitor–based and anti-estrogenic therapies, while PIK3CA mutations have high prevalence in MBC. As a r...

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Literature Corpus work
e9c3718d-d138-5c2b-8a57-8d162b38527c
DOI
10.1101/598847
Open publication

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