Article

Genomic in vitro transcription and Nanopore direct RNA sequencing of a human B-Lymphocyte cell line

2025-04-27

Abstract excerpt

Genomic DNA used as a template for in vitro transcription of RNA can serve as a true negative control for benchmarking RNA modification detection by Nanopore direct RNA sequencing (DRS) models. We generated DRS data for in vitro transcribed (IVT) RNA composed of canonical nucleotides using genomic DNA from a human cell line. We applied Dorado modification calling models to these data, and calculated 9-mer specific...

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Identifiers and source

Literature Corpus work: e81bb9dc-22ee-5233-a318-25e86df586d1
DOI: 10.1101/2025.04.25.650674

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