Article

Targeted sequencing of <i>Enterobacterales</i> bacteria using CRISPR-Cas9 enrichment and Oxford Nanopore Technologies

2024-06-27

Abstract excerpt

Sequencing DNA directly from patient samples enables faster pathogen characterisation compared to traditional culture-based approaches, but often yields insufficient sequence data for effective downstream analysis. CRISPR-Cas9 enrichment is designed to improve yield of low abundance sequences but has not been thoroughly explored with Oxford Nanopore Technologies (ONT) for use in clinical bacterial epidemiology. We...

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Literature Corpus work: 98a3af58-71af-53ed-b9ee-fbbf7f7e929c
DOI: 10.1101/2024.06.26.600727

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