Article

Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs

2016-08-05

Abstract excerpt

<h4>ABSTRACT</h4> Changes in small non-coding RNAs such as micro RNAs (miRNAs) can serve as indicators of disease and can be measured using next-generation sequencing of RNA (RNA-seq). Here, we highlight the need for approaches that complement RNA-seq, discover that northern blotting of small RNAs is biased against short sequences, and develop a protocol that removes this bias. We found that multiple small RNA-se...

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Literature Corpus work: db97724e-9fe6-5584-a914-14fbb5775ac8
DOI: 10.1101/068031

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