CRISPR-HOLMES-based NAD ⁺ detection

Abstract excerpt

Studies have indicated that intracellular nicotinamide adenine dinucleotide (NAD + ) level is associated with the occurrence and development of many diseases. While traditional NAD + detection techniques are time-consuming and may require large and expensive instruments. We recently found that the CRISPR-Cas12a protein can be inactivated by AcrVA5-mediated acetylation and reactivated by CobB, using NAD + as the...

Topics

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• Biosensing Techniques
• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• DNA
• Gene Editing
• Polymorphism, Single Nucleotide
• RNA

Identifiers and source

Literature Corpus work

d61f8437-ad0c-51ed-86f1-5ae5a1526733

DOI

10.1101/2023.12.06.570494