Article

Genome-wide Spatial Expression Profiling in FFPE Tissues

2020-07-25

Abstract excerpt

Formalin-fixed paraffin embedding (FFPE) is the most widespread long-term tissue preservation approach. Here we present a procedure to perform genome-wide spatial analysis of mRNA in FFPE tissue sections. The procedure takes advantage of well-established, commercially available methods for imaging and spatial barcoding using slides spotted with barcoded oligo(dT) probes to capture the 3’ end of mRNA molecules in t...

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Literature Corpus work: d2468227-5f04-56f4-a252-81c625a308db
DOI: 10.1101/2020.07.24.219758

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