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Article

Genome-wide spatial expression profiling in formalin-fixed tissues

1 Dec 2021

Abstract excerpt

Formalin-fixed paraffin embedding (FFPE) is the most widespread long-term tissue preservation approach. Here, we report a procedure to perform genome-wide spatial analysis of mRNA in FFPE-fixed tissue sections, using well-established, commercially available methods for imaging and spatial barcoding using slides spotted with barcoded oligo(dT) probes to capture the 3' end of mRNA molecules in tissue sections. We...

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PMID: 36776149
DOI: 10.1016/j.xgen.2021.100065

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