Article

Rapid identification of maternal lineages in common carp (Cyprinus carpio L.) using real-time PCR and high resolution melt-curve analysis

2009-02-01

Abstract excerpt

This study presents a protocol for using real-time PCR and high-resolution melt-curve (HRMC) analysis to score polymorphisms in the mitochondrial DNA control region of common carp. This is the first time HRMC analysis has been used in an aquacultural species. The technique is accurate, robust and rapid to apply. It has a number of advantages over other existing techniques for rapidly scoring DNA polymorphisms, nam...

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Literature Corpus work: d107732a-4505-5103-9e3a-e1d27ff73cd3
DOI: 10.1016/j.aquaculture.2008.10.035

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