Article
High-resolution melt analysis without DNA extraction affords rapid genotype resolution and species identification.
Molecular ecology resources - 1 Jul 2017
Rugman-Jones Paul F, Stouthamer Richard
Abstract excerpt
Extracting and sequencing DNA from specimens can impose major time and monetary costs to studies requiring genotyping, or identification to species, of large numbers of individuals. As such, so-called direct PCR methods have been developed enabling significant savings at the DNA extraction step. Similarly, real-time quantitative PCR techniques (qPCR) offer very cost-effective alternatives to sequencing....
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