Article
Rapid screening for Native American mitochondrial and Y-chromosome haplogroups detection in routine DNA analysis.
Forensic science international. Genetics - 1 Mar 2011
Zuccarelli Gala, Alechine Evguenia, Caputo Mariela, Bobillo Cecilia, Corach Daniel, Sala Andrea
Abstract excerpt
Aiming to detect individuals of Native American maternal or paternal ancestry a rapid screening approach has been developed. Its strategy was based on SNP typing by Real Time PCR (rt-PCR) followed by High Resolution Melting analysis (HRM). After extraction, DNA was quantitated by rt-PCR using commercial kits; samples were then submitted to two multiplex reactions in order to determine the major Native American...
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