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Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and the 16S-ITS-23S of the rrn operon

2019-08-01

Abstract excerpt

<h4>Background: </h4> Profiling the microbiome of low-biomass samples is challenging for metagenomics since these samples are prone to contain DNA from other sources (e.g. host or environment). The usual approach is sequencing short regions of the 16S rRNA gene, which fails to assign taxonomy to genus and species level. To achieve an increased taxonomic resolution, we aim to develop long-amplicon PCR-based approac...

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Literature Corpus work
8a47d6bb-c887-51b9-acc1-96c8c27d8a6c
DOI
10.12688/f1000research.16817.2
Open publication

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Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and the 16S-ITS-23S of the rrn operonDOI 10.12688/f1000research.16817.2
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