Article
Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and the 16S-ITS-23S of the rrn operon
2019-08-01
Abstract excerpt
<h4>Background: </h4> Profiling the microbiome of low-biomass samples is challenging for metagenomics since these samples are prone to contain DNA from other sources (e.g. host or environment). The usual approach is sequencing short regions of the 16S rRNA gene, which fails to assign taxonomy to genus and species level. To achieve an increased taxonomic resolution, we aim to develop long-amplicon PCR-based approac...
Topics
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- Bacterial Identification and Susceptibility Testing
- Clostridium difficile and Clostridium perfringens research
- Dermatology and Skin Diseases
- Gut microbiota and health
- Infections and bacterial resistance
- Infective Endocarditis Diagnosis and Management
- Mycobacterium research and diagnosis
- Orthopedic Infections and Treatments
Identifiers and source
- Literature Corpus work
- 8a47d6bb-c887-51b9-acc1-96c8c27d8a6c
- DOI
- 10.12688/f1000research.16817.2
