Article

Quantitative PCR provides a simple and accessible method for quantitative microbiome profiling

2018-11-27

Abstract excerpt

The use of relative next generation sequencing (NGS) abundance data can lead to misinterpretations of microbial community structures as the increase of one taxon leads to concurrent decrease of the other(s). To overcome compositionality, we provide a quantitative NGS solution, which is achieved by adjusting the relative 16S rRNA gene amplicon NGS data with quantitative PCR (qPCR-based) total bacterial counts. By c...

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Literature Corpus work: 06a9b47a-13ba-5c3b-9624-6e364ac184e1
DOI: 10.1101/478685

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