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Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements

28 Apr 2021

Abstract excerpt

BACKGROUND: Validation and standardization of methodologies for microbial community measurements by high-throughput sequencing are needed to support human microbiome research and its industrialization. This study set out to establish standards-based solutions to improve the accuracy and reproducibility of metagenomics-based microbiome profiling of human fecal samples. RESULTS: In the first phase, we performed a...

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PMID: 33910647
DOI: 10.1186/s40168-021-01048-3

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