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Establishment of a Method for Describing stx Genes Expression of E. coli O157:H7 in Ground Beef Matrix during Refrigerated Storage

2016-05-01

Abstract excerpt

Ingredients and complexity of food matrices are inhibitory factors for gene expression studies of bacterial pathogens directly from food matrix. The aim of this study was to develop a simple and fast method for RNA extraction from ground beef matrix as well as stx genes expression of Escherichia coli O157:H7 by relative quantitative real‐time reverse transcription‐polymerase chain reaction (RT‐PCR). Inoculated gro...

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Literature Corpus work
cc77a703-afd3-5895-bcaf-67da0c4e5570
DOI
10.1111/jfs.12234
Open publication

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Establishment of a Method for Describing stx Genes Expression of E. coli O157:H7 in Ground Beef Matrix during Refrigerated StorageDOI 10.1111/jfs.12234
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