Article

The HIV-1 provirus excised by a single CRISPR/Cas9 RNA guide persists in the host cell and may be reactivated

2020-11-17

Abstract excerpt

Gene editing may be used to cut out the human immunodeficiency virus type-1 (HIV-1) provirus from the host cell genome and eradicate infection. Here, using cells acutely or latently infected by HIV and treated with long terminal repeat-targeting CRISPR/Cas9, we show that the excised HIV provirus persists for a few weeks and, by means of HIV Integrase, rearranges in circular molecules. Circularization and integrati...

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Literature Corpus work: c6ef6d3f-e008-5ff9-897b-6a71b8b2cea4
DOI: 10.1101/2020.11.16.384180

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