Article

Accurate quantitation of 16S gene copies in low biomass samples post-antibiotic treatment through deep sequencing with a balanced nucleotide synthetic spike-in approach

2024-09-28

Abstract excerpt

The microbiota significantly impacts health and treatment outcomes. While 16S rRNA gene sequencing reveals relative bacterial abundances, it does not provide absolute quantification. We developed a cost-effective solution incorporating synthetic DNA standards designed to ensure balanced nucleotide representation at each position. These standards are spiked into samples before DNA extraction, enabling simultaneous...

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Literature Corpus work: c364cc1f-65df-50d7-bdef-d86a5ad70473
DOI: 10.1101/2024.09.27.615519

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