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Highly accurate and sensitive absolute quantification of bacterial strains in human fecal samples

7 Sept 2024

Abstract excerpt

Next-generation sequencing (NGS) approaches have revolutionized gut microbiome research and can provide strain-level resolution, but these techniques have limitations in that they are only semi-quantitative, suffer from high detection limits, and generate data that is compositional. The present study aimed to systematically compare quantitative PCR (qPCR) and droplet digital PCR (ddPCR) for the absolute...

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Share to Clostridium difficile and Clostridium perfringens research
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PMID: 39244633
DOI: 10.1186/s40168-024-01881-2

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