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Article

Adjusting microbiome profiles for differences in microbial load by spike-in bacteria

21 Jun 2016

Abstract excerpt

BACKGROUND: Next-generation 16S ribosomal RNA gene sequencing is widely used to determine the relative composition of the mammalian gut microbiomes. However, in the absence of a reference, this does not reveal alterations in absolute abundance of specific operational taxonomic units if microbial loads vary across specimens. RESULTS: Here we suggest the spiking of exogenous bacteria into crude specimens to...

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PMID: 27329048
DOI: 10.1186/s40168-016-0175-0

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