Back to search

Article

Systematic analysis of factors that improve HDR efficiency in CRISPR / Cas9 technique

2020-01-24

Abstract excerpt

<title>Abstract</title> <p>The bacterial CRISPR/Cas9 system has a proven to be an efficient tool for genetic manipulation in various organisms, but the efficiency of sequence replacement by homologous direct repair (HDR) is substantially lower than random creation of indels. Many studies focused on improving the efficiency of HDR using double sgRNA, cell synchronization cycle and the delivery of ssODN with a rati...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
bfa77224-16f0-560e-aae4-905f3852e285
DOI
10.21203/rs.2.21769/v1
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Systematic analysis of factors that improve HDR efficiency in CRISPR / Cas9 techniqueDOI 10.21203/rs.2.21769/v1
Systematic analysis of factors that improve homologous direct repair (HDR) efficiency in CRISPR/Cas9 technique.Semantic proximity
Rational Design of ssODN to Correct Mutations by Gene Editing.Semantic proximity
An Update on the Application of CRISPR Technology in Clinical PracticeSemantic proximity
Alternative strategy to induce CRISPR-mediated genetic changes in hematopoietic cellsSemantic proximity
Harnessing the potential of gene editing technology using CRISPR in inflammatory bowel diseaseSemantic proximity
Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair.Semantic proximity
Technical considerations for the use of CRISPR/Cas9 in hematology researchSemantic proximity
Profiling the impact of the promoters on CRISPR-Cas12a system in human cellsSemantic proximity
Amelioration of hemophilia B through CRISPR/Cas9 induced homology-independent targeted integrationSemantic proximity
<i>In vitro</i> and <i>In vivo</i> Genetic Disease Modelling via NHEJ-precise deletions using CRISPR/Cas9Semantic proximity
Development of gene editing strategies for human β-globin (HBB) gene mutations.Semantic proximity
Development of Gene Editing Strategies for Human <b>β</b> -Globin (HBB) Gene MutationsSemantic proximity
Select a neighboring publication to make it the new centre.