Article

Integrated genetic screening reveals FEN1 as a driver of stemness and temozolomide resistance in glioblastoma

2025-06-24

Abstract excerpt

Glioblastoma (GBM) is a lethal brain tumor with limited response to standard of care chemoradiotherapy. In this study, we conducted genome-wide CRISPR knockout screening in patient-derived glioblastoma stem cells (GSCs) to identify genetic dependencies of cell survival and therapy resistance. Our screening identified flap endonuclease 1 (FEN1) as a key driver of GSC survival, with enhanced dependency under temozol...

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Literature Corpus work: bf65305b-00b4-5fd1-8316-309d906d36ca
DOI: 10.1101/2025.06.18.660339

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