Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substrates

Abstract excerpt

Deubiquitylating enzymes (DUBs) counteract ubiquitylation to control stability or activity of substrates. Identification of DUB substrates is challenging because multiple DUBs act on the same substrates, thwarting genetic approaches. Here, we circumvented redundancy by broadly inhibiting DUBs in Xenopus egg extract. DUB inhibition increases ubiquitylation of hundreds of proteins, depleting free ubiquitin without...

Topics

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• Antineoplastic Agents
• Autophagy in Disease and Therapy
• Endopeptidases
• Endoplasmic Reticulum Stress and Disease
• Genetics and Neurodevelopmental Disorders
• Histone Deacetylase Inhibitors Research
• Peptidase Inhibition and Analysis
• Ubiquitin and proteasome pathways

Identifiers and source

Literature Corpus work

bced2ab4-af7b-57f3-9355-bfb2ae4b1955

DOI

10.1101/844811