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Analysis of the ARTIC V4 and V4.1 SARS-CoV-2 primers and their impact on the detection of Omicron BA.1 and BA.2 lineage-defining mutations.

Microbial genomics - 1 Apr 2023

Ulhuq Fatima R, Barge Madhuri, Falconer Kerry, Wild Jonathan, Fernandes Goncalo, Gallagher Abbie, McGinley Suzie, Sugadol Ahmad, Tariq Muhammad, Maloney Daniel, Kenicer Juliet, Dewar Rebecca, Templeton Kate, McHugh Martin P

Abstract excerpt

The ARTIC protocol uses a multiplexed PCR approach with two primer pools tiling the entire SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) genome. Primer pool updates are necessary for accurate amplicon sequencing of evolving SARS-CoV-2 variants with novel mutations. The suitability of the ARTIC V4 and updated V4.1 primer scheme was assessed using whole genome sequencing of Omicron from clinical...

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PMID: 37083576
DOI: 10.1099/mgen.0.000991
PMCID: PMC10210939

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