Article

Evaluation of in-house molecular assays for detection of HIV-1 proviral DNA

2025-08-06

Abstract excerpt

HIV-1 proviral DNA is a critical marker for the early diagnosis of vertical transmission, especially when viral load RNA is suppressed by antiretrovirals. Our objective was to evaluate the performance of a qualitative qPCR to detect HIV-1 proviral DNA. Using the TaqMan system, the assay targets the Integrase (IN), Long Terminal Repeat (LTR) regions of HIV-1, and the CCR5 gene as an internal control. To improve sen...

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Literature Corpus work: b0a94822-70fd-5e36-8e8a-7318e9f1eeca
DOI: 10.1101/2025.07.31.25332407

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