Article

A comparison of six DNA extraction protocols for 16S, ITS, and shotgun metagenomic sequencing of microbial communities

2022-03-08

Abstract excerpt

Microbial communities contain a broad phylogenetic diversity of organisms, however the majority of methods center on describing bacteria and archaea. Fungi are important symbionts in many ecosystems, and are potentially important members of the human microbiome, beyond those that can cause disease. To expand our analysis of microbial communities to include fungal ITS data, we compared five candidate DNA extraction...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: b04d86f9-87bb-5c8a-a8ea-48d2c216e6e0
DOI: 10.1101/2022.03.07.483343

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A comparison of six DNA extraction protocols for 16S, ITS, and shotgun metagenomic sequencing of microbial communitiesDOI 10.1101/2022.03.07.483343Read

Assessment of fecal DNA extraction protocols for metagenomic studiesSemantic proximity Read

A unique and reliable fecal DNA extraction method for 16S rRNA gene and shotgun metagenomic sequencing in the analysis of the human gut microbiomeSemantic proximity Read

Comparison of DNA extraction methods for 16S rRNA gene sequencing in the analysis of the human gut microbiomeSemantic proximity Read

Evaluating the Impact of DNA Extraction Method on the Representation of Human Oral Bacterial and Fungal CommunitiesSemantic proximity Read

Evaluation of fecal DNA extraction protocols for human gut microbiome studiesSemantic proximity Read

Toward Standards in Clinical Microbiota Studies: Comparison of Three DNA Extraction Methods and Two Bioinformatic PipelinesSemantic proximity Read

Assessment of DNA extraction methods for human gut mycobiome analysisSemantic proximity Read

Toward standards in clinical microbiome studies: comparison of three DNA extraction methods and two bioinformatic pipelinesSemantic proximity Read

A simple, cost-effective and automation-friendly direct PCR approach for bacterial community analysisSemantic proximity Read

Optimisation of a DNA extraction protocol for improving the bacterial and fungal classification based on Nanopore sequencingSemantic proximity Read

The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community RecoverySemantic proximity Read

A Comparison of Three Automated Nucleic Acid Extraction Systems for Human Stool SamplesSemantic proximity Read