Article

CRISPR screen reveals that EHEC’s T3SS and Shiga toxin rely on shared host factors for infection

2018-05-08

Abstract excerpt

Enterohemorrhagic Escherichia coli (EHEC) has two critical virulence factors – a type III secretion system (T3SS) and Shiga toxins (Stx) – that are required for the pathogen to colonize the intestine and cause diarrheal disease. Here, we carried out a genome-wide CRISPR/Cas9 loss-of-function screen to identify host loci that facilitate EHEC infection of intestinal epithelial cells. Many of the guide RNAs identifi...

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Literature Corpus work: afc94cd2-d7e5-5e31-9b89-398299c587c0
DOI: 10.1101/316919

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