Article

MetaPlex: An Ion Torrent COI metabarcoding workflow and toolkit to increase experimental efficacy and efficiency

2022-09-15

Abstract excerpt

DNA barcoding has become a dependable way to assign taxonomic identifications to otherwise unknown DNA samples. Metabarcoding using next-generation sequencing (NGS) maintains this same caliber of taxonomic identification and can occur on a mass scale in pooled, or multiplexed, samples from a wide variety of sources when properly multiplexed. However, this methodology has several pitfalls, including inaccurate sour...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: aeaee8c3-a9d7-5105-8b48-0e3ccb61e348
DOI: 10.22541/au.166322714.48673632/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

MetaPlex: An Ion Torrent COI metabarcoding workflow and toolkit to increase experimental efficacy and efficiencyDOI 10.22541/au.166322714.48673632/v1Read

Ion torrent high throughput mitochondrial genome sequencing (HTMGS).Semantic proximity Read

Phylogenetic Authentication of Amplicon Sequence Variants in Single-Specimen Metabarcoding of Tropical Insects.Semantic proximity Read

Metabarcoding mosquitoes: MinION sequencing of bulk samples gives accurate species profiles for vector surveillance (Culicidae)Semantic proximity Read

Computational Evaluation of DNA Metabarcoding for Universal Diagnostics of Invasive Insect PestsSemantic proximity Read

MBIOME: A comprehensive, reproducible, and open-source workflow for amplicon-based microbiome data analysisSemantic proximity Read

A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers.Semantic proximity Read

Assessment of Common and Emerging Bioinformatics Pipelines for Targeted Metagenomics.Semantic proximity Read

Comparing library preparation methods for SARS-CoV-2 multiplex amplicon sequencing on the Illumina MiSeq platformSemantic proximity Read

Illumina But With Nanopore: Sequencing Illumina libraries at high accuracy on the ONT MinION using R2C2Semantic proximity Read

Optimizing illumina next-generation sequencing library preparation for extremely at-biased genomesSemantic proximity Read

<i>De novo</i> clustering of long-read amplicons improves phylogenetic insight into microbiome dataSemantic proximity Read

Single laboratory evaluation of the (Q20+) nanopore sequencing kit for bacterial outbreak investigationsSemantic proximity Read