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Article

Phylogenetic Authentication of Amplicon Sequence Variants in Single-Specimen Metabarcoding of Tropical Insects.

Molecular ecology resources - 1 Jul 2026

Ounjai Sarawut, Liu Huaxi, Zhou Zichen, Correia Maria Pestana, Creedy Thomas J, Andújar Carmelo, Arribas Paula, Vogler Alfried P

Abstract excerpt

High-throughput sequencing (HTS) allows large-scale DNA barcoding of individually tagged specimens ('megabarcoding'), but deep amplicon sequencing produces a mixture of authentic mitochondrial sequences together with nuclear pseudogenes (NUMTs), environmental and cross-sample contaminants, and sequencing artefacts. Standard approaches relying on read clustering or dominant-read selection often fail to classify...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 42420666
DOI: 10.1111/1755-0998.70178
PMCID: PMC13346335

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

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