Article

Substrate stiffness modulates integrin α5 expression and ECM-associated gene expression in fibroblasts

2021-11-22

Abstract excerpt

Biomaterials, like polydimethylsiloxane (PDMS), are soft, biocompatible, and tuneable, which makes them useful to delineate specific substrate factors that regulate the complex landscape of cell-substrate interactions. We used a commercial formulation of PDMS to fabricate substrates with moduli 40 kPa, 300 kPa, and 1.5 MPa, and cultured HMF3S fibroblasts on them. Gene expression analysis was performed by RT-PCR an...

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Literature Corpus work: ac964016-df1f-5a3b-a419-228ff10b62f8
DOI: 10.1101/2021.11.22.469526

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