Metabolic engineering improves transduction efficiency and downstream vector isolation by altering the lipid composition of extracellular vesicle-enclosed AAV.

Abstract excerpt

Adeno-associated viruses (AAV) are promising vectors for gene therapy due to their efficacy in vivo. However, there is room for improvement to address key limitations such as the pre-existing immunity to AAV in patients, high-dose toxicity, and relatively low efficiency for some cell types. This study introduces a metabolic engineering approach, using knockout of the enzyme phosphatidylserine synthase 1 (PTDSS1) t...

Topics

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• CAR-T cell therapy research
• Extracellular vesicles in disease
• MicroRNA in disease regulation
• Parvovirus B19 Infection Studies
• RNA Interference and Gene Delivery
• Viral Infections and Immunology Research
• Viral Infectious Diseases and Gene Expression in Insects
• Virus-based gene therapy research

Identifiers and source

Literature Corpus work

ac4a1bc7-a848-5839-abf8-a548f0a2a838

DOI

10.1101/2024.08.16.608303