Article

A Novel Propidium Monoazide-Based PCR Assay Can Measure Viable Uropathogenic <i>E. coli in Vitro</i> and <i>in Vivo</i>

2021-10-13

Abstract excerpt

<h4>Background</h4> Polymerase chain reaction (PCR) is an important means by which to study the urine microbiome and is emerging as possible alternative to urine cultures to identify pathogens that cause urinary tract infection (UTI). However, PCR is limited by its inability to differentiate DNA originating from viable, metabolically active versus non-viable, inactive bacteria. This drawback has led to concerns t...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a953512c-2f99-5d04-9f1d-30aa9627283e
DOI: 10.1101/2021.10.13.464198

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A Novel Propidium Monoazide-Based PCR Assay Can Measure Viable Uropathogenic <i>E. coli in Vitro</i> and <i>in Vivo</i>DOI 10.1101/2021.10.13.464198Read

Improving clinical significance of PCR: Use of propidium monoazide to distinguish viable from dead <i>staphylococcus aureus</i> and <i>staphylococcus epidermidis</i>Semantic proximity Read

Engineered reporter phages for rapid detection of <i>Escherichia coli, Klebsiella</i> spp., and <i>Enterococcus</i> spp. in urineSemantic proximity Read

Whole microbial community viability is not quantitatively reflected by propidium monoazide sequencing approachSemantic proximity Read

Dead or Alive? Molecular life-dead distinction in human stool samples reveals significantly different composition of the microbial communitySemantic proximity Read

<i>Cryptosporidium</i> Propidium Monoazide-PCR, a Molecular Biology-Based Technique for Genotyping of Viable <i>Cryptosporidium</i> OocystsSemantic proximity Read

Population Dynamics and Niche Distribution of Uropathogenic Escherichia coli during Acute and Chronic Urinary Tract InfectionSemantic proximity Read

Removal of Contaminant DNA by Combined UV-EMA Treatment Allows Low Copy Number Detection of Clinically Relevant Bacteria Using Pan-Bacterial Real-Time PCRSemantic proximity Read

<i>Escherichia coli</i> from Urine of Female Patients with Urinary Tract Infections Is Competent for Intracellular Bacterial Community FormationSemantic proximity Read

A rapid ionic liquid-based DNA extraction method for molecular diagnostics of urinary tract infectionsSemantic proximity Read

Sensitive and rapid detection of pathogenic bacteria from urine samples using multiplex recombinase polymerase amplificationSemantic proximity Read

Uropathogenic E. coli induces DNA damage in the bladderSemantic proximity Read

Variability in cell division among anatomical sites shapes Escherichia coli antibiotic survival in a urinary tract infection mouse modelSemantic proximity Read