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<i>Cryptosporidium</i> Propidium Monoazide-PCR, a Molecular Biology-Based Technique for Genotyping of Viable <i>Cryptosporidium</i> Oocysts

12 Sept 2009

Abstract excerpt

Cryptosporidium is an important waterborne protozoan parasite that can cause severe diarrhea and death in the immunocompromised. The current methods used to monitor for Cryptosporidium oocysts in water are the microscopy-based USEPA methods 1622 and 1623. These methods assess total levels of oocysts in source waters, but do not determine oocyst viability or genotype. Recently, propidium monoazide (PMA) has been...

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PMID: 19749067
DOI: 10.1128/aem.00540-09

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