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A guide to selecting high-performing antibodies for GCase (UniProt ID: P04062) for use in western blot, immunoprecipitation, and immunofluorescence

2025-12-15

Abstract excerpt

The human GBA1 gene encodes glucocerebrosidase (GCase), a lysosomal enzyme that hydrolyzes glucosylceramides. Variants in GBA1 and reduced GCase activity have been linked to Parkinson’s disease and Gaucher’s disease. Here we have characterized twenty-four GCase commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-ou...

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Literature Corpus work
a1d536fb-651e-5e76-85c6-3fe15309f909
DOI
10.12688/f1000research.174230.1
Open publication

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