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A guide to selecting high-performing antibodies for ASM (UniProt ID: P17405) for use in western blot and immunoprecipitation

2026-04-28

Abstract excerpt

The SMPD1 gene encodes the acid sphingomyelinase (ASM) protein, a lysosomal enzyme essential for hydrolysing sphingomyelin into ceramide and phosphorylcholine, a key step in lipid metabolism and membrane homeostasis. Here we have characterized nine ASM commercial antibodies for western blot and immunoprecipitation using a standardized experimental protocol based on comparing read-outs in knockout cell lines and i...

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Literature Corpus work
781f8067-bdf7-5915-a09f-82bc9d10b1ac
DOI
10.12688/f1000research.178749.1
Open publication

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