Article
Identification of two heme-binding sites in the cytoplasmic heme-trafficking protein PhuS from Pseudomonas aeruginosa and their relevance to function.
Biochemistry - 18 Dec 2007
Block Darci R, Lukat-Rodgers Gudrun S, Rodgers Kenton R, Wilks Angela, Bhakta Mehul N, Lansky Ila B
Abstract excerpt
PhuS is a cytoplasmic, 39 kDa heme-binding protein from Pseudomonas aeruginosa. It has previously been shown to transfer heme to its cognate heme oxygenase. It is expressed from the phu operon, which encodes a group of proteins known to actively internalize and transport heme from host organisms. This study combines the spectral resolution of resonance Raman spectroscopy with site-directed mutagenesis to identify...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.