Article

Deletion of a GA-repeat in human GPM6B disrupts differentiation of NT2 cells to neural cells

2023-12-02

Abstract excerpt

Across multiple primates and tissues, GPM6B (Glycoprotein membrane 6B) reaches maximum levels of expression in the human brain. This gene contains a strictly monomorphic short tandem repeat (STR) of (GA)9. We used a CRISPR-based tool to delete this GA-repeat in NT2 cells, and analyzed the consequence of this deletion on GPM6B expression, using qRT-PCR and western blot. Subsequently, the edited cells were induced t...

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Literature Corpus work: a083a826-72ab-596a-88cd-5a09b4fb311f
DOI: 10.21203/rs.3.rs-3658367/v1

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