A comparison of the phenotypic and genetic stability of recombinant Trichoderma spp. generated by protoplast- and Agrobacterium-mediated transformation.

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Article

A comparison of the phenotypic and genetic stability of recombinant Trichoderma spp. generated by protoplast- and Agrobacterium-mediated transformation.

Journal of microbiology (Seoul, Korea) - 1 Aug 2006

Cardoza Rosa Elena, Vizcaino Juan Antonio, Hermosa Maria Rosa, Monte Enrique, Gutiérrez Santiago

Abstract excerpt

Four different Trichoderma strains, T. harzianum CECT 2413, T. asperellum T53, T. atroviride T11 and T. longibrachiatum T52, which represent three of the four sections contained in this genus, were transformed by two different techniques: a protocol based on the isolation of protoplasts and a protocol based on Agrobacterium-mediated transformation. Both methods were set up using hygromycin B or phleomycin...

Topics

Agrobacterium tumefaciens
DNA, Fungal
Drug Resistance, Fungal
Genetic Markers
Genome, Fungal
Genomics
Hygromycin B
Phenotype
Phleomycins
Protoplasts
Transformation, Genetic
Trichoderma

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Open in PubMed

Identifiers

PMID: 16953173

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