Article
A CRISPR-Cas9 System for Genome Editing of Fusarium proliferatum.
Scientific reports - 27 Dec 2019
Ferrara Massimo, Haidukowski Miriam, Logrieco Antonio F, Leslie John F, Mulè Giuseppina
Abstract excerpt
Fusarium proliferatum causes diverse diseases of many economically important plants. The fungus produces several mycotoxins of which the fumonisins are the most toxic. Currently, deletion of key genes for mycotoxin biosynthesis is a laborious and time-consuming procedure. We developed a novel CRISPR/Cas9-based genome-editing tool for the direct delivery of preassembled Cas9 ribonucleoproteins into protoplasts of...
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