Article

PBMC Fixation and Processing for Chromium Single-Cell RNA Sequencing

2018-05-05

Abstract excerpt

<h4>Background</h4> Interest in single-cell transcriptomic analysis is growing rapidly, especially for profiling rare or heterogeneous populations of cells. In almost all reported works investigators have used live cells, which introduces cell stress during preparation and hinders complex study designs. Recent studies have indicated that cells fixed by denaturing fixative can be used in single-cell sequencing, ho...

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Literature Corpus work: 95494f7b-5b32-5423-8b8d-417d9b766401
DOI: 10.1101/315267

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