Article

Sample-multiplexed FACS-preprocessing of PBMCs enables scalable scRNA-seq without compromising transcriptomic or cellular integrity

2025-11-07

Abstract excerpt

Efficient preprocessing of peripheral blood mononuclear cells (PBMCs) for single-cell RNA-Sequencing (scRNA-seq) is crucial to ensure high sample throughput while maintaining sample integrity. In particular, when enrichment of rare immune cell populations is necessary to enable their representative profiling among more common PBMCs, sample preprocessing may become a detrimental bottleneck. Here, we present an opti...

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Literature Corpus work: f324c0ac-e129-51d8-9ba3-facbbdf7028e
DOI: 10.1101/2025.11.06.687014

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