Article

Cryo-PRO facilitates whole blood cryopreservation for single-cell RNA sequencing of immune cells from clinical samples

2024-09-19

Abstract excerpt

<h4>ABSTRACT</h4> Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) has enhanced our understanding of host immune mechanisms in small cohorts, particularly in diseases with complex and heterogeneous immune responses such as sepsis. However, standard PBMC isolation from blood requires technical expertise and over two hours of onsite processing using Ficoll density gradient separa...

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Literature Corpus work: 703fb9fd-c5c8-5027-81e2-57fe1589ed04
DOI: 10.1101/2024.09.18.24313760

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