Article

Click Detect: A Rapid and Sensitive Assay for Shiga Toxin 2 Detection

2025-10-15

Abstract excerpt

Shiga toxin–producing Escherichia coli (STEC) are major foodborne pathogens responsible for severe gastrointestinal disease and hemolytic uremic syndrome (HUS). Here we report Click Detect, a novel diagnostic platform that leverages click display – an in vitro protein display technology – to generate uniform and covalently linked protein–cDNA conjugates for toxin detection. Using a DARPin (D#20) as the signal prob...

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Literature Corpus work: 92722db1-fd99-5ed0-b976-bb915c1aa093
DOI: 10.20944/preprints202510.1085.v1

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