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Article

O-antigen and virulence profiling of shiga toxin-producing Escherichia coli by a rapid and cost-effective DNA microarray colorimetric method.

Frontiers in cellular and infection microbiology - 1 Jan 2012

Quiñones Beatriz, Swimley Michelle S, Narm Koh-Eun, Patel Ronak N, Cooley Michael B, Mandrell Robert E

Abstract excerpt

Shiga toxin-producing Escherichia coli (STEC) is a leading cause of foodborne illness worldwide. The present study developed the use of DNA microarrays with the ampliPHOX colorimetric method to rapidly detect and genotype STEC strains. A low-density 30-mer oligonucleotide DNA microarray was designed to target O-antigen gene clusters of 11 E. coli serogroups (O26, O45, O91, O103, O104, O111, O113, O121, O128,...

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Identifiers

PMID: 22919652
DOI: 10.3389/fcimb.2012.00061
PMCID: PMC3417394

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Source receipt: pubmed, retrieved 7 Sept 2026.

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