Article

Mass allelic exchange: enabling sexual genetics in <i>Escherichia coli</i>

2020-12-01

Abstract excerpt

Despite dramatic advances in genomics, connecting genotypes to phenotypes is still challenging. Sexual genetics combined with linkage analysis is a powerful solution to this problem but generally unavailable in bacteria. We build upon a strong negative selection system to invent Mass Allelic Exchange (MAE), which enables hybridization of arbitrary (including pathogenic) strains of E. coli . MAE reimplements the n...

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Literature Corpus work: 8e4249c2-6eea-522d-ae2c-91f991851bf0
DOI: 10.1101/2020.11.30.405282

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