Article

A Transfection-Free Approach of Gene Editing via a gold-based nanoformulation of the Cas9 protein

2024-07-10

Abstract excerpt

In recent years, the CRISPR/Cas9 technology has emerged as a highly efficient tool for cell gene editing. However, the delivery of the CRISPR/Cas9 system into cells remains a significant challenge, drastically limiting in vivo gene therapy applications. In this study, we present a transfection/transduction-free tool for intracellular delivery of the Cas9:gRNA ribonucleoprotein. The Cas9 enzyme is conjugated to a...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8a18bc9e-bd1b-5a9e-820c-3b49a22c06cf
DOI: 10.1101/2024.07.09.602746

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A Transfection-Free Approach of Gene Editing via a gold-based nanoformulation of the Cas9 proteinDOI 10.1101/2024.07.09.602746Read

Advances in Nanoparticles as Non-Viral Vectors for Efficient Delivery of CRISPR/Cas9Semantic proximity Read

Cas9 RNP Physiochemical Analysis for Enhanced CRISPR-AuNP Assembly and FunctionSemantic proximity Read

CRISPR-Cas9 mediated nuclear transport and genomic integration of nanostructured genes in human primary cellsSemantic proximity Read

Packaging and Uncoating of CRISPR/Cas Ribonucleoproteins for Efficient Gene Editing with Viral and Non-viral Extracellular NanoparticlesSemantic proximity Read

Aptamer-Guided Gene Therapy for Cancer DiseaseSemantic proximity Read

A Recombinant Targeted Bionanoparticle In Vitro Expressing a Gene-Editing Protein for Epidermal Growth Factor Receptor Mutant Lung Cancer.Semantic proximity Read

Non-viral nucleic acid containing nanoparticles as cancer therapeuticsSemantic proximity Read

Fighting RNA viruses with a gold nanoparticle Cas13d gene-editing armorSemantic proximity Read

Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skippingSemantic proximity Read

Carboxylated nanodiamond-mediated CRISPR-Cas9 delivery of human retinoschisis mutation into human iPSCs and mouse retina.Semantic proximity Read

A modular strategy for extracellular vesicle-mediated CRISPR-Cas9 delivery through aptamer-based loading and UV-activated cargo releaseSemantic proximity Read

Optimized nanoparticle-mediated delivery of CRISPR-Cas9 system for B cell interventionSemantic proximity Read