Article

Real-time TIRF imaging of single adiponectin vesicle exocytosis in 3T3-L1 adipocytes stably expressing mCherry fused to human adiponectin

2022-09-14

Abstract excerpt

Adiponectin is a peptide hormone abundantly released from adipocytes, and reduced circulating levels are associated with obesity-related diseases, such as type 2 diabetes, insulin resistance and cardiovascular disease. Adiponectin is released by regulated exocytosis of secretory vesicles, but traditional molecular biology and imaging techniques lack the specificity and time resolution to adequately quantify exocyt...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 89512d8b-e4e4-5b90-b0f8-7faea4a669ba
DOI: 10.1101/2022.09.13.507762

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Real-time TIRF imaging of single adiponectin vesicle exocytosis in 3T3-L1 adipocytes stably expressing mCherry fused to human adiponectinDOI 10.1101/2022.09.13.507762Read

Intracellular trafficking and secretion of adiponectin is dependent on GGA-coated vesicles.Semantic proximity Read

Live-cell imaging identifies cAMP microdomains regulating β-adrenoceptor-subtype-specific lipolytic responses in human white adipocytesSemantic proximity Read

Local protein dynamics during microvesicle exocytosis in neuroendocrine cells.Semantic proximity Read

Insulin regulates fusion of GLUT4 vesicles independent of Exo70-mediated tethering.Semantic proximity Read

Extracellular vesicles as predictors of individual response to exercise training in youth living with obesitySemantic proximity Read

Imaging constitutive exocytosis with total internal reflection fluorescence microscopy.Semantic proximity Read

Algorithm for automatic detection of insulin granule exocytosis in human pancreatic β-cellsSemantic proximity Read

Exocytosis of post-Golgi vesicles is regulated by components of the endocytic machinery.Semantic proximity Read

GLUT4 is sorted to vesicles whose accumulation beneath and insertion into the plasma membrane are differentially regulated by insulin and selectively affected by insulin resistance.Semantic proximity Read

Phosphorylation of the exocyst protein Exo84 by TBK1 promotes insulin-stimulated GLUT4 trafficking.Semantic proximity Read

Expression of a synapsin IIb site 1 phosphorylation mutant in 3T3-L1 adipocytes inhibits basal intracellular retention of Glut4.Semantic proximity Read

Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore.Semantic proximity Read