Article

redkmer: an assembly-free pipeline for the identification of abundant and specific X-chromosome target sequences for X-shredding by CRISPR endonucleases

2017-09-15

Abstract excerpt

CRISPR-based synthetic sex ratio distorters, that operate by shredding the X-chromosome during male meiosis, are promising tools for the area-wide control of harmful insect pest or disease vector species. However, the selection of gRNA targets, in the form of high-copy sequence repeats on the X chromosome of a given species, is difficult since such repeats are not accurately resolved in genome assemblies and can’t...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8556764a-6620-516f-b192-eb2c6c1f5915
DOI: 10.1101/189431

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

redkmer: an assembly-free pipeline for the identification of abundant and specific X-chromosome target sequences for X-shredding by CRISPR endonucleasesDOI 10.1101/189431Read

Spermatogenic context controls outcomes of engineered sex distortion in malaria mosquitoesSemantic proximity Read

A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiaeSemantic proximity Read

SPANDx: a genomics pipeline for comparative analysis of large haploid whole genome re-sequencing datasets.Semantic proximity Read

Measuring the Impact of Genetic Heterogeneity and Chromosomal Inversions on the Efficacy of CRISPR-Cas9 Gene Drives in Different Strains of Anopheles gambiaeSemantic proximity Read

Single-molecule sequencing of long DNA molecules allows high contiguity de novo genome assembly for the fungus fly, Sciara coprophilaSemantic proximity Read

Measuring the impact of genetic heterogeneity and chromosomal inversions on the efficacy of CRISPR-Cas9 gene drives in different strains of Anopheles gambiaeSemantic proximity Read

CRISPR-Cas9 and Cas12a target site richness reflects genomic diversity in natural populations of Anopheles gambiae and Aedes aegypti mosquitoes.Semantic proximity Read

The development of gene drives for genetic control of the malaria mosquitoSemantic proximity Read

The beginning of the end: a chromosomal assembly of the New World malaria mosquito ends with a novel telomereSemantic proximity Read

Cas9-mediated maternal effect and derived resistance alleles in a gene-drive strain of the African malaria vector mosquito, Anopheles gambiae.Semantic proximity Read

High-specificity detection of rare alleles with Paired-End Low Error Sequencing (PELE-Seq).Semantic proximity Read

Potential Opportunities and Challenges of Deploying Next Generation Sequencing and CRISPR-Cas Systems to Support Diagnostics and Surveillance Towards Malaria Control and Elimination in AfricaSemantic proximity Read