Article

Cell freezing protocol optimized for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

2016-01-15

Abstract excerpt

In recent years, the assay for transposase-accessible chromatin using sequencing (ATAC-Seq) has become a fundamental tool of epigenomic research. However, it has proven difficult to perform this technique on frozen samples because freezing cells before extracting nuclei impairs nuclear integrity and alters chromatin structure. We describe a protocol for freezing cells that is compatible with ATAC-Seq, producing re...

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Literature Corpus work: 7edcbefd-af89-5d97-9f39-1dc6682fe0bc
DOI: 10.1101/036798

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