Article

A sample-to-report solution for taxonomic identification of cultured bacteria in the clinical setting based on nanopore sequencing

2019-08-31

Abstract excerpt

Amplicon sequencing of 16S rRNA gene is commonly used for the identification of bacterial isolates in diagnostic laboratories, and mostly relies on the Sanger sequencing method. The latter, however, suffers from a number of limitations with the most significant being the inability to resolve mixed amplicons when closely related species are co-amplified from a mixed culture. This often leads to either increased tur...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 7ceb3344-52bf-5b04-a978-aaf03c137b63
DOI: 10.1101/752774

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A sample-to-report solution for taxonomic identification of cultured bacteria in the clinical setting based on nanopore sequencingDOI 10.1101/752774Read

Performance and Practicality of 16S Nanopore Sequencing for Routine Bacterial Identification in Clinical SamplesSemantic proximity Read

Standardization of 16S rRNA gene sequencing using nanopore long read sequencing technology for clinical diagnosis of culture negative infectionsSemantic proximity Read

Nationwide multicentre study of Nanopore long-read sequencing for 16S rRNA-species identificationSemantic proximity Read

Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technologySemantic proximity Read

Understanding and overcoming the pitfalls and biases of next-generation sequencing (NGS) methods for use in the routine clinical microbiological diagnostic laboratorySemantic proximity Read

Evaluation of long-read 16S rRNA next-generation sequencing for identification of bacterial isolates in a clinical diagnostic laboratorySemantic proximity Read

Optimizing and Evaluating Nanopore-Based Targeted and Metagenomic Sequencing Workflows for Rapid Diagnosis of Acute Invasive Infections from Normally Sterile Body FluidsSemantic proximity Read

Real-time Nanopore de novo assembly of ESKAPE pathogens: Implications for antibiotic stewardshipSemantic proximity Read

High-accuracy meets high-throughput for microbiome profiling with near full-length 16S rRNA amplicon sequencing on the Nanopore platformSemantic proximity Read

Improving the Diagnosis of Bacterial Infections: Evaluation of 16S rRNA Nanopore Metagenomics in Culture-Negative SamplesSemantic proximity Read

Nanopore sequencing enables highly accurate genotyping and identification of resistance determinants in key nosocomial pathogensSemantic proximity Read

Nanopore sequencing enables highly accurate genotyping and identification of resistance determinants in key nosocomial pathogens.Semantic proximity Read